However, neither SIRT5 knockdown nor overexpression significantly affected migration (Fig. 5J) or proliferation (Supplementary Fig. 1B) in the K163R mutant group, suggesting that SIRT5 specifically recognizes and deacetylates the K163 site on MRPL12, thereby promoting the malignant phenotype of ccRCC cells. This evidence concerns the gene SIRT5 and nonpapillary renal cell carcinoma.