Additionally, GFAT1 was highly expressed in mouse model of HCC induced by diethylnitrosamine (DEN) and carbon tetrachloride (CCl4), as well as in a mouse model utilizing hydrodynamic tail vein injection of Sleeping Beauty (SB) transposase-based plasmids expressing SB100/AKT/NRAS (Fig. 1J, K). This evidence concerns the gene GFPT1 and hepatocellular carcinoma.