Based on the identified glycosylation modified peptide of VEZF1 (119-134), sequence containing S123 and S124 (V1) or the S123A and S124A mutations (V2), we designed cell-penetrating peptides (CPPs) to introduce a competitive inhibitor of VEZF1 O-GlcNAcylation into HCC cells. Here, VEZF1 is linked to hepatocellular carcinoma.