G3BP1/G3BP2 double-knockout (dKO) U2OS cells was further constructed, which were rescued with G3BP1-WT, G3BP1-N258A, and G3BP1-N309A to further validate that the cleavage sites at N258 and N309 of G3BP1 actually occurred in tumor cells during drug treatment (Fig. S4h and S4i). Here, G3BP1 is linked to neoplasm.