To further investigate the role of PRMT1 in gastric cancer (GC), we successfully established stable GC cell lines (HCG27, AGS, and MFC) with PRMT1 knockdown, overexpression, and enzyme activity mutations using lentiviral vectors to transduce short hairpin RNA (shRNA) and PRMT1 enzyme mutant plasmids (Fig. 1H, I, Supplementary Fig. 1D, E, supplementary Fig. 2A–C, supplementary Fig. 2K–M and supplementary Fig. 2U). This evidence concerns the gene PRMT1 and gastric cancer.