Chloroquine (CQ), an autophagic lysosome inhibitor, can inhibit lysosomal activity, and the addition of CQ to some extent exacerbated the increase in the LC3II expression induced by shACSS2 (Fig. 3C, D), indicating that ACSS2 knockout inhibited autophagy flux in HNSCC cells. Here, ACSS2 is linked to head and neck squamous cell carcinoma.