Similarly, after overexpression of TFEB, the expression of LC3II in shACSS2 HNSCC, while the expression of SQSTM1 did not show a significant increase (Fig. 7A–D), and the fluorescence intensities of DQ-BSA and LysoSensor Green were significantly enhanced, indicating that the lysosomal function and autophagic flux of head and neck squamous cell carcinoma cells were partially improved (Fig. 7E–H). Here, TFEB is linked to head and neck squamous cell carcinoma.