Consistent with HER2/HER3 activation, recombinant ASPN induced activating phosphorylations on AKT, ERK, and calcium pathway members (PLCγ, calcium/calmodulin-dependent protein II [CAMKII]) in human and mouse prostate cancer cells (Figure 2, A–D, and Supplemental Figure 2, A–D). Here, ASPN is linked to prostate cancer.