In addition, CD4+ T cell subset analysis in tumor-infiltrating cells showed Th1 and Th17 cells increased significantly more in the mice that received the E743–77-pulsed bm12 mBMDCs than mice that received either the E743–77-pulsed WT or bm1 mBMDC vaccines, but levels of Th2 and Tfh cells and Tregs were unchanged (Figure 2H; gating strategy, and Supplemental Figure 4). This evidence concerns the gene CD4 and neoplasm.