To determine whether depleting Foxp3+ Tregs starting at the later stage of the tumor growth enhanced the antitumor activity of the MHC class II semiallogeneic bm12 mBMDC vaccine, we used B6.129(Cg)-Foxp3tm3(Hbegf/GFP)Ayr/J (Foxp3-GFPDTR) mice, which undergo selective depletion of Foxp3+ cells upon administration of diphtheria toxin (DT) (42). This evidence concerns the gene HBEGF and neoplasm.