CD8A and neoplasm: The tumor growth curves of the combinations with early and late Treg depletion crossed at 23 days after TC-1 inoculation, so we analyzed the percentage of intratumoral E743–77-specific CD8+ T cells and Foxp3+ Tregs, as well as those in spleen and blood, 23 days after TC-1 inoculation when there was no difference in tumor size to affect the results (Figure 6, D–G; gating strategy, and Supplemental Figure 9).