Additionally, Syk and other downstream molecules, such as Grb2, Cdc42, Pak1, Nfkb1, and Nfkb2, followed a similar expression pattern following IRI (Figure 8J and Supplemental Figure 19), consistent with coordinated inflammatory signal activation within macrophages during AKI-to-CKD transition. This evidence concerns the gene PAK1 and chronic kidney disease.