MAP7D1 and Shwachman-Diamond syndrome: Since SDS is an autosomal recessive disorder, endogenous MAP7D1 expression was silenced using siRNA before overexpressing either the wild-type or the mutant MAP7D1 version in HEK293T cells (Fig. 6C), to account for the potential compensatory effect of endogenously expressed wild-type MAP7D1 protein on the overexpressed mutant version of MAP7D1 and, thus, to better observe the effect of the mutation on mitosis.