Considering the important regulatory role of macrophages in inflammatory responses in sepsis, we extracted mouse peritoneal macrophages and co‐incubated them with supernatants of primary hepatocytes from GSDMDflox+/+ mice and GSDMDhep‐/− mice, as well as supernatants of primary hepatocytes from GSDMDhep‐/− mice injected with AAV‐GSDMD and AAV‐GSDMD‐c.D276A. Here, GSDMD is linked to Sepsis.