Detection of DUX4 mRNA in FSHD muscle cells had been so difficult up to this point that a reviewer requested a point to point comparison of conditions used for RNA extraction and RT-PCR alongside the methodology used by the 6 previous publications that could not detect DUX4 transcripts [52, 94, 102, 107, 123, 124]. The gene discussed is DUX4; the disease is facioscapulohumeral muscular dystrophy.