This study aimed to assess the feasibility and performance of hSPECT/LiDAR in analyzing tissue specimens excised after robotic <sup>99m</sup>Tc-PSMA-radioguided surgery.<h4>Methods</h4>We included samples from 5 prostate cancer patients undergoing primary or salvage robot-assisted resection of <sup>99m</sup>Tc-PSMA-I&S avid lesions that were identified using a drop-in gamma probe. The gene discussed is FOLH1; the disease is prostate cancer.