We first created an interferon (IFN)-alpha/beta receptor alpha chain (IFNAR1)-deficient human embryonic kidney 293 (HEK293) cell line (clone 14) via CRISPR-Cas9 to eliminate the confounding effects of type I IFN signaling on viral infection and propagation (Fig. S1A and B). The gene discussed is IFNAR1; the disease is viral infectious disease.