PLCB4 and glioblastoma: We validated these findings through a meta-analysis that integrated RNA-seq (n = 1,817) and microarray (n = 1,151) datasets from LGG and GBM samples, confirming that low PLCB4 expression was associated with poor overall survival (HR = 1.32, 95% CI: 1.11–1.57, p< 0.001) (Supplementary Figure S3).