ACTA2 and liver cancer: We observed similar expression levels of representative liver cancer differentiation markers (AFP and ALB), proliferation marker (Ki67), tight junction marker (CDH1), epithelial-to-mesenchymal transition (EMT), and metastasis-related markers (ACTA2 and PDGFRB) between xenografts established with Matrigel and Liver ECM (Fig. 1D).