The resultsfrom both methods suggest the formation of an HCC–HSA complex;however, the stability of this complex appears to be relatively low.Further analysis of the ITC data indicated that the interaction betweenHSA and HCC is primarily driven by the increase of entropy upon binding(the TΔS component of Gibbsfree energy was −9.99 kcal/mol, and enthalpy was 1.88 kcal/mol).Additionally, the binding stoichiometry, calculated using a singleset of identical site binding model, was found to be 0.642. Here, ALB is linked to hepatocellular carcinoma.