Although both HSA and HCCproteins are present in blood plasma and cerebrospinal fluid in highconcentrations, the possibility of their interactions was only speculated,and the complexation process between them has not yet been characterized.To study HSA–HCC direct interactions, we employed differentcomplementary techniques such as affinity chromatography, mass spectrometry,MST, and ITC. The gene discussed is ALB; the disease is hepatocellular carcinoma.