The collected 2D 1H–15N HSQC spectraof the uniformly 15N-labeled V57G HCC demonstrated no visibleshifts in the position of the signals originating from various amidegroups of HCC (Figure ), indicating that the 3D structure of the V57G HCC mutant does notchange upon the addition of equimolar amounts of HSA. This evidence concerns the gene ALB and hepatocellular carcinoma.