Conversely, trehalose induced autophagy via a phosphoinositide kinase-FYVE finger containing (PIKFYVE)- Transient receptor potential channel mucolipin-1 (TRPML1)-dependent pathway, promoting TFEB nuclear translocation, upregulating autophagy and lysosomal genes, enhancing phagosome-lysosome colocalization, and thus restricting intracellular mycobacterial survival during both single and HIV co-infection (Sharma V. et al., 2021). Here, PIKFYVE is linked to coinfection.