In both the GSE8401 dataset (primary and metastatic melanoma)[25] and the GSE65904 dataset (metastatic melanoma),[26]COL1A1 expression was significantly negatively correlated with CTL infiltration, suggesting that higher collagen levels are associated with reduced CD8+ T cell presence in human melanomas (Figure8A,B). This evidence concerns the gene CD8A and metastatic melanoma.