CTNNB1 and hepatocellular carcinoma: A hydrodynamic transfection-based mouse model of HCC was established by hydrodynamic transfection with pCMV/SB11, pT3-EF1a-c-Met, pT3-N90-beta-catenin, LentiCRISPR-sgP53, and LentiCRISPR-sgPten plasmids, following previously reported protocols [16].