By utilizing the previously described thyroid cancer cell lines, we found that B7-H3 had a more homogenous and intense expression than CSPG4 (100% and 84 ± 22% of positive stained cells with a mean fluorescence intensity of 68106 and 483930 for B7-H3 and CSPG4, respectively) (Fig. 4B, C). This evidence concerns the gene CD276 and thyroid cancer.