To investigate the in vitro antitumor activity of CSPG4-targeted CAR T cells, we first assessed CSPG4 membrane expression on 4 human thyroid cancer cell lines, including 8505c and SW1736 (ATC), 2 C-HCC (hurthle cell carcinoma) and BCPAP (PTC). Here, CSPG4 is linked to thyroid cancer.