To increase the number of antigen-specific T-cells, HLA-A*02:01 positive PBMCs (healthy control n = 6) or BMMCs (AML patients n = 6) were stimulated with WT1 peptides and then re-stimulated with peptide-loaded CD14+ monocytes in the presence of cytokines (experimental schema Fig. 1A). Here, HLA-A is linked to acute myeloid leukemia.