The Reactome pathway analysis based on GSEA (Fig. 6F) revealed that the PBMCs of individuals with obesity mainly participated in pathways related to lipid metabolism, G Protein-Coupled Receptor (GPCR) signaling, general metabolism, GPCR downstream signaling, Receptor tyrosine kinases (RTK) signaling, RNA polymerase II transcription, generic transcription pathways, gene expression (transcription), hemostasis, and the adaptive immune system. The gene discussed is ACKR3; the disease is obesity due to melanocortin 4 receptor deficiency.