Both mutants exhibited significant virulence attenuation compared with the wild-type and DAL81 complemented strains (Fig. 8D), as we found that mice infected with the wild-type strain had a median survival time of 4 days, whereas survival times for those infected with the mutant strains remained undefined due to prolonged survival, indicating that Dal81 and Stp2 are required for virulence in this infection model. Here, SULT1A2 is linked to infection.