Furthermore, in agreement with previous studies (Burrell et al, 2013; Bohly et al, 2019, 2022), we detected reduced fork progression rates and thus endogenous mild replication stress in CIN+ cancer cells, but treatment with Wnt10b or Wnt3a did not grossly affect replication fork progression dynamics in these cells (Fig 4F) or in RPE1-hTERT cells treated with aphidicolin (Fig S4C), indicating that activation of Wnt signaling does not alleviate replication stress. This evidence concerns the gene WNT3A and cancer.