VCP and amyotrophic lateral sclerosis: To test whether SFPQ is alternatively spliced during motor neuron development in the context of ALS, we examined splicing in our poly(A) RNA-seq dataset of VCP mutation–related ALS (VCP-ALS hereafter) compared to CTRL samples undergoing motor neurogenesis (25), with a focus on LSV chr1:35187001-35187122.