We observed a general trend of higher methylation levels in blood leukocytes of the HD group than in controls and patients with LC for 33 CpG sites in 12 genes (PSEN1, SETDB1, TWIST1, HSPA4, HDAC1, NFKB1, GLS2, MIR10B, SP1, GRIA2, BCL11B, and LEP). The gene discussed is HSPA4; the disease is laryngotracheoesophageal cleft.