At the end of the BAK induction phase (day 7), prior to treatment initiation, corneal whole mounts from all groups revealed a nearly complete loss of nerve fibers (Figure S1), confirming that BAK exposure reliably induced severe corneal denervation, which is consistent with its well-documented neurotoxic effects in dry eye models.81 The gene discussed is BAK1; the disease is Keratoconjunctivitis sicca.