TYMS and colorectal carcinoma: Similar trends were found for TS protein levels with 5-FU and CF10 treatment [Supplementary Figure 5], but the effects were larger at the protein level (~7-fold) than for TYMS mRNA (~2-fold) in HCT116R cells [Figure 3A-C] and the other CRC cell lines selected for 5-FU/LV-resistance [Supplementary Figures 1-4].