Alongside MYC, the C2 MKI67+ TCs subpopulation had elevated expression of stemness-associated genes, including EZH2, NOTCH1, and CD44. EZH2 is recognized as a significant biomarker in various malignancies, including colorectal and prostate cancer (59), and it has been shown to boost the growth and dissemination of CRC cells (60). Here, NOTCH1 is linked to colorectal carcinoma.