NOTCH1 and Familial prostate cancer: Alongside MYC, the C2 MKI67+ TCs subpopulation had elevated expression of stemness-associated genes, including EZH2, NOTCH1, and CD44. EZH2 is recognized as a significant biomarker in various malignancies, including colorectal and prostate cancer (59), and it has been shown to boost the growth and dissemination of CRC cells (60).