To investigate the relationship between RAB5B expression and TME, a comprehensive evaluation of the matrix index, immune index, and ESTIMATE score was conducted across several tumor types.The results showed that there was a significant negative statistical correlation between the expression level of RAB5B and matrix index, immune index and ESTIMATE score, especially in these tumors with high scores, and only a few cancers showed positive correlation, such as COAD and DLBC (Figure 7A). This evidence concerns the gene RAB5B and cancer.