In addition, the protein expression of vascular endothelial growth factor (VEGF) was measured on 7th and 14th days respectively, and we found that G-Rb1 can effectively promote angiogenesis after CIRI, while XAV939 co-treated with G-Rb1 can delay angiogenesis after cerebral ischemia. This evidence concerns the gene PIK3R1 and Cerebral ischemia.