Both BuShenHuaZhuo formula and YulinTongBu formula can reduce the levels of lipopolysaccharide (LPS) and inflammatory cytokines such as TNF-α, IL-6 and IL-8 in the serum of PCOS rats, and inhibit the inflammatory response mediated by the nuclear factor-κB (NF-κB) signaling pathway (48, 50). This evidence concerns the gene TNF and polycystic ovary syndrome.