DCs are known to instruct tumor Ag-reactive T cells, including CD8POS T cells (primarily through subsets such as conventional DC1s and, to a lesser extent, plasmacytoid DCs [pDCs]), to proliferate and activate their cytotoxic machinery.42 When CD8POS T cells isolated from GL261 tumors were co-cultured with naive DCs, no effect in the rIL-12 condition (bottom image) was observed, compared with Fc sham control (top image), as assessed by IFN-γ secretion (Figure S4C). This evidence concerns the gene IFNG and neoplasm.