Markers were predominantly co-expressed in tumor-associated CD4 T cells, CD8 T cells, and natural killer cells (annotated as the NK/T cells cluster); however, in datasets with broader immune representation (as these datasets were enriched with CD45POS cells), expression was also observed in macrophages, monocytes, microglia, and DCs in the TME of de novo and recurrent glioma (Figure S3A). Here, CD4 is linked to central nervous system cancer.