STAT4 and glioma: IL-12p70 binds to the dimeric receptor composed of the IL-12 receptor β1 (IL12Rβ1) and β2 (IL12Rβ2) subunits, leading to phosphorylation of Tyr693 on the receptor-associated STAT4 transcription factor.39 This phosphorylation promotes STAT4 dimerization, thereby initiating pro-inflammatory signaling.31,40 To identify the cells capable of an IL-12p70-mediated anti-GB effect, we analyzed available human glioma scRNA-seq datasets (Table S2) for the expression of relevant genes (IL12Rβ1, Il12Rβ2, and STAT4) (Figure 2A).