Furthermore, significant interaction effect was observed for G6Pase (P < 0.0001, 32.21% lower in T2D+HIIT compared with T2D).Furthermore, PEPCK showed higher levels in the T2D group compared to the CON (P < 0.0001, 17.32% higher) and the T2D+HIIT (P < 0.0001, 60.23% change) ones. The gene discussed is PCK2; the disease is type 2 diabetes mellitus.