While the anti-leukemic activity was maintained, exposure to allogeneic NK cells led to an increase in PD-L1 expression on the remaining blasts, albeit to a very heterogeneous extent between the AML samples, with a trend for a further increase in the presence of AFM28; minor effects were observed for HLA-I (Supplementary Fig. 4A–C). Here, CD274 is linked to acute myeloid leukemia.