But its role in breast cancer remains unclear.<h4>Objective</h4>The purpose of this research is to construct the sh-RANBP1 cell line derived from a human breast cancer cell line in order to investigate the impact of low RANBP1 expression on the expression network of breast cancer-associated miRNAs and mRNAs.<h4>Methods</h4>We constructed the sh-RANBP1 cell line from the human breast cancer cell line MDA-MB-231 and analyzed the significant differential expression between the two groups using miRNA sequencing and mRNA sequencing of both the sh-RANBP1 group and the control group. This evidence concerns the gene RANBP1 and breast cancer.