First, to determine the efficiency of MEN1-KD upon the treatment of siMEN1#1 and siMEN1#2 in BLCA cells, we analyzed the MEN1 mRNA and menin protein expression levels in siMEN1#1 or siMEN1#2-treated cancer cells by RT-qPCR and western blotting assay. Here, MEN1 is linked to bladder transitional cell carcinoma.