Using marker genes obtained from the CellMarker 2.0 database, we annotated seven major cell types: epithelial cells (EPCAM, KRT18, KRT19, CLDN4), NKT cells (NKG7, KLRD1, GNLY, CD3D, CD3E, CD2), B cells (MS4A1, CD79A, CD79B, IGHG1), macrophages (SSP1, CD68, IL1B, CD163), cancer-associated fibroblasts (CAFs; COL1A2, COL3A1, DCN, THY1), endothelial cells (PECAM1, VWF, CDH5, PLVAP), and mast cells (CPA3, GATA2, MS4A2, TPSB2) (Figure 5B). Here, CLDN4 is linked to cancer.