In the present study, a hypoxia- and immune-associated prognostic signature, which included 6 genes (GALR2, AGT, MAPT, PRKCG, CAMK2B, and PKP1), was established via LASSO Cox regression in patients with pancreatic cancer from the TCGA dataset and was further validated in 2 independent GEO datasets. This evidence concerns the gene PRKCG and pancreatic neoplasm.