Using the same 4T1 tumor mouse model with the same treatment schedule, we excised tumors right after treatment (Fig. 5A) to assess TILs, including CD4 + T cells, CD8 + T cells, macrophages, monocytic-myeloid-derived suppressor cells (M-MDSCs), and regulatory T cells (Tregs) under various treatment conditions. This evidence concerns the gene CD8A and neoplasm.