Both invitro and in vivo experimental results demonstrated that P-Fe3O4@Pal@HM nanomaterials, under NIR irradiation,efficiently targeted and killed osteosarcoma cells and their HVECs.This effect was achieved by inducing apoptosis through ROS generationand RRM2 inhibition, resulting in enhanced antitumor efficacy. The gene discussed is RRM2; the disease is osteosarcoma.