Importantly, our imaging analysis could readily identify changes in host factor recruitment and spatial proximity at vDNA under a variety of genetic (e.g., NTC vs. PML or Daxx KO; Figs 5 and 6) and infection (WT vs. ΔICP0 HSV-1; Fig 8) conditions, demonstrating the sensitivity of the approach employed. The gene discussed is PML; the disease is infection.