TWIST1 and breast cancer: Conversely, using shRNA, we obtained an ∼70% knockdown (KD) of snail in the invasive breast cancer BT549 cells (Neve et al., 2006; SenGupta et al., 2021b) (Supplemental Figure S1E) and a depletion of twist in M2, M4, and BT549 twist knockout (KO) cell lines (Supplemental Figure S1, Fi–iii).