These computational analyses further substantiate the lack of an association between the regulation of CXCL8 and CXCL1 expressions and EMT-associated changes in breast cancer cells and confirm the existence of a TGF-β/TNF-α regulatory axis for CXCL1 and CXCL8 that is, again, independent of EMT induction in breast cancer cells. The gene discussed is CXCL1; the disease is breast cancer.