TBK1 and breast cancer: To further confirm whether this prometastatic effect of TBK1 is specifically contributed by TAMs, we used shRNA-mediated knockdown system to deplete TBK1 in 4T1 cells (SI Appendix, Fig. S2K) and found that TBK1 depletion in breast cancer cells did not affect their EMT status, migration, and invasion ability (SI Appendix, Fig. S2 L–N).