As a result, we found that depletion of CSF2 or CCL2 in tumor-associated THP1 and Raw264.7 macrophages reduced EMT as shown by the reduced expression of mesenchymal makers vimentin and N-cadherin and upregulated expression of epithelial marker E-cadherin in MDA-MB-231-Br and 4T1 cells (Fig. 5D and SI Appendix, Fig. S5 E and F). This evidence concerns the gene CCL2 and neoplasm.