To investigate the effects of H. pylori (particularly the role of CagA as a key virulence factor) and EBV co-infection on GC, we used NGOs stably established in vitro as a “host” model with a high-accuracy microinjection technique to introduce different H. pylori strains of SS1, NCTC11637, and NCTC12908 (carrying with or without CagA) in the presence or absence of EBV virion particles into the lumen of organoids and visualize subsequent growth of the organoids for 24 h after infection. Here, S100A8 is linked to infection.