The integrated query (Online MethodsQuery 10) completed in seconds and returned 27 genes including known cardiac developmental regulators, including GATA4, GATA5, GATA6, NKX2-5, TBX20, CITED2, CREBBP, and EP300, all with established roles in septation, chamber specification, and congenital heart defects 56–62. The gene discussed is NKX2-5; the disease is congenital heart disease.