The in-depth exploration of the pathological mechanisms of osteosarcoma and its related signaling pathways, the development and metastasis of osteosarcoma are closely related to several cell signaling pathways, including Wnt/β-catenin, PI3K/Akt, RAF/MEK/ERK and mTOR (34), which is consistent with the results of this study. Here, AKT1 is linked to osteosarcoma.