Combined with the PPI network in this study, it is hypothesized that Solasonine may synergistically regulate the “stress-responsive-molecular chaperone cluster”(HSP90AA1, HSPA8, HSP90B1) and the”metabolism-invasive synergistic network”(TPI1, MMP2) (38, 39), which could promote apoptosis and inhibit proliferation of osteosarcoma cells, and enhance the sensitivity of chemotherapy. This evidence concerns the gene TPI1 and osteosarcoma.