On the basis of the WB results, we conducted real-time fluorescence quantitative PCR experiments on samples from normal individuals and IBD patients for the second time and found that DUOX2 (P = 0.0253), LCN2 (P = 0.00873), and DEFA6 (P = 0.00348) still demonstrated outstanding discrimination, whereas LOC389023 performed poorly (P = 0.114), which may be related to its nature as a long noncoding RNA. Here, DEFA6 is linked to inflammatory bowel disease.