Xenotransplantation and fluorescence-activated cell sorting (FACS) experiments have identified key indicators for CSCs in HCC, which can be categorized into intracellular indicators, like cytokeratin 19 (CK19), and surface indicators, such as CD13, CD24, CD44, CD47, CD90, CD133, and epithelial cellular adhesion molecule (EpCAM)8. The gene discussed is PROM1; the disease is hepatocellular carcinoma.