ACHE and Cognitive impairment: The effects of POF on cholinergic system components, including AChE, have not been reported, but it is well known that ovarian hormones have an important role in learning and memory, and deprivation from these hormones has an important role in cognitive impairment and cholinergic dysfunction (Balderan et al., 2023; Craig et al., 2010; Hejazian et al., 2016).